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The isoelectric point (pI) is the pH at which a molecule carries no net electrical charge. The Isoelectric Point Plugin calculation considers all macro ionization states of an ionizable molecule across the pH range. The net charge at given pH is calculated from the weighted sum of the macro distribution.
Proteins have minimal solubility at their isoelectric point. The mobility of molecule is minimal at pI and so an important parameter for electroanalytical separation techniques.
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